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Monitoring Pyridoxine by Capillary Zone Electrophoresis with Electrochemical Detection
Author(s) -
Jin Wenrui,
Zhang Jie
Publication year - 2000
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/(sici)1521-4109(20000401)12:6<465::aid-elan465>3.0.co;2-r
Subject(s) - capillary electrophoresis , detection limit , chromatography , amperometry , chemistry , analytical chemistry (journal) , electrophoresis , capillary action , microelectrode , relative standard deviation , electrochemistry , electrode , materials science , composite material
Capillary zone electrophoresis was employed for the determination of pyridoxine using end‐column amperometric detection with a carbon fiber microelectrode at a constant potential of 0.72 V (vs. SCE). The optimum conditions of separation and detection are 1.0×10 –2 mol/L NH 4 Cl–1.0×10 –2 mol/L NH 3 for the buffer solution, 10 kV for the separation voltage, 5 kV and 10 s for the injection voltage and the injection time, respectively. The limit of detection is 1.2×10 –6 mol/L or 3.5 fmol ( S/N =2). The relative standard deviation is 1.3 % for the migration time and 3.4 % for the electrophoretic peak current. The method was applied to determining pyridoxine in human urine.