Premium
Amine Oxidase Based Amperometric Biosensors forHistamine Detection
Author(s) -
Niculescu Mihaela,
Frébort Ivo,
Peč Pavel,
Galuszka Petr,
Mattiasson Bo,
Csöregi Elisabeth
Publication year - 2000
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/(sici)1521-4109(20000301)12:5<369::aid-elan369>3.0.co;2-j
Subject(s) - biosensor , detection limit , amperometry , chemistry , linear range , electrode , electron transfer , amine gas treating , selectivity , analytical chemistry (journal) , chromatography , electrochemistry , organic chemistry , biochemistry , catalysis
This work reports on the development and optimization of amperometric biosensors based on the enzyme amine oxidase (AO) for the detection of histamine, a well‐known biomarker for food freshness. Biosensor characteristics were evaluated in a flow injection (FI) analysis line, operated at +200 mV (vs. Ag/AgCl/0.1 M KCl). Two different biosensor designs were considered, one based on adsorbed AO on graphite electrodes, the detection being based on a direct electron transfer (DET) mechanism, whereas the second one based on an Os‐bipyridine modified redox polymer using a mediated electron transfer (MET) pathway. Both electrode designs were able to detect histamine in µM range, however, the [osmium(4,4′‐dimethylbipyridine) 2 Cl] +/2+ complexed with poly(1‐vinylimidazole) (PVI 13 ‐dmeOs) based electrodes showed superior characteristics with regard to stability, selectivity and linear range. These electrodes were characterized by a detection limit of 2.2 µM (calculated as three times the signal‐to‐noise ratio), a sensitivity of 6.8 mA M –1 cm –2 , a linear range of 10–200 µM, and an operational stability of 20 % response loss during 8 h of continuous operation at a sample throughput of 30 injections h –1 .