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A Reagentless Amperometric Carbon Paste Based Sensor for NADH
Author(s) -
Malinauskas Albertas,
Ruzgas Tautgirdas,
Gorton Lo,
Kubota Lauro T.
Publication year - 2000
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/(sici)1521-4109(200002)12:3<194::aid-elan194>3.0.co;2-6
Subject(s) - amperometry , detection limit , electrode , anode , chemistry , inorganic chemistry , electrochemistry , nile blue , chromatography , physics , quantum mechanics , fluorescence
Carbon paste electrodes, based on zirconium phosphate with immobilized N ‐methylphenazonium or Nile blue, were prepared. The electrodes show anodic current response to NADH at an operating potential ranging from –0.05 to 0.30 V (vs. SCE). The sensitivity of electrodes to NADH was shown to increase with increasing operation potential. It was shown that the concentration dependences for electrodes, obtained at different operating potentials, follow Michaelis‐Menten scheme, confirming an electrocatalytic mechanism for electrooxidation of NADH with a precursor complex formation. The detection limit for NADH was found to be ca. 2 µM for both types of sensors.