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Determination of Dihydrozeatin and Dihydrozeatin Riboside by Cathodic Stripping Voltammetry
Author(s) -
Blanco Manuel H.,
Quintana María del Carmen,
Hernández Lucas
Publication year - 2000
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/(sici)1521-4109(200002)12:2<147::aid-elan147>3.0.co;2-f
Subject(s) - cathodic stripping voltammetry , detection limit , chemistry , stripping (fiber) , chromatography , electrolyte , voltammetry , buffer solution , analytical chemistry (journal) , electrochemistry , electrode , materials science , composite material
The present study develops methods to quantify the phytohormones dihydrozeatin (DHZ) and dihydrozeatin ridoside (DHZR) using cathodic stripping and differential pulse voltammetry (DPS). Employing a supportive electrolyte with 0.04 M acetic/acetate buffer pH 4.5 and 40 s accumulation time at –0.60 V, the detection and determination limits for DHZ were 5.5 and 8.0 ng mL –1 , respectively. For DHZR using a 0.04 M acetic/acetate buffer pH 4 with a 50 s accumulation time at –0.95 V the detection limit was 16.0 ng mL –1 with a determination limit of 24.0 ng mL –1 . Both methods were successfully applied to determine minute amounts of cytokinins in apples.