Premium
Microfabricated Conductometric Urea Biosensor Based on Sol‐Gel Immobilized Urease
Author(s) -
Lee WonYong,
Lee Kang Shin,
Kim TaeHan,
Shin MinChol,
Park JeKyun
Publication year - 2000
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/(sici)1521-4109(20000101)12:1<78::aid-elan78>3.0.co;2-b
Subject(s) - urease , biosensor , urea , chemistry , chromatography , conductometry , hydrolysis , bovine serum albumin , immobilized enzyme , buffer solution , nuclear chemistry , biochemistry , enzyme
A conductometric biosensor was constructed with a sol‐gel immobilized urease on a microfabricated interdigitated array (IDA) gold electrode. Tetramethoxysilane (TMOS) was used as a sol‐gel precursor for the immobilization of the urease and the performance of the resulting urea biosensor was controlled through the water content in the acid‐catalyzed hydrolysis of the sol‐gel stock solution. The sol‐gel‐derived urea biosensor showed relatively wide dynamic range of 0.2–50 mM in 5 mM imidazole‐HC1 buffer at pH 7.5. Furthermore, the urea biosensor exhibited good sensor‐to‐sensor reproducibility (6.5 %) and storage stability (50 % of its original activity retained after 3 weeks) when stored in 5 mM imidazole‐HC1 buffer at 4 °C. The urea biosensor was successfully applied for the urea determination in urine sample by employing the differential measurement format consisting of an active IDA conductometric sensor containing sol‐gel‐immobilized urease and a reference IDA conductometric sensor containing bovine serum albumin layer instead of the urease.