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Detection of Interaction Between Metal Complex Indicator and DNA by Using Electrochemical Biosensor
Author(s) -
Erdem Arzum,
Meric Burcu,
Kerman Kagan,
Dalbasti Tayfun,
Ozsoz Mehmet
Publication year - 1999
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/(sici)1521-4109(199912)11:18<1372::aid-elan1372>3.0.co;2-4
Subject(s) - dna , carbon paste electrode , chemistry , electrochemistry , biosensor , cationic polymerization , electrode , metal , hybridization probe , salt (chemistry) , ligand (biochemistry) , carbon fibers , inorganic chemistry , combinatorial chemistry , nuclear chemistry , cyclic voltammetry , polymer chemistry , organic chemistry , biochemistry , materials science , receptor , composite material , composite number
There has been extensive research on binding of transition metal complexes to DNA via electrostatic and hydrophobic interactions. Most indicator based electrochemical DNA biosensors have used cationic metal complexes that interact in a different way with single‐stranded DNA (ssDNA) and double‐stranded DNA (dsDNA). Described here are the electrochemical parameters for a mixed‐ligand complex, [Co(phen) 3 3+ ] (phen: 1,10‐phenanthroline), on binding to DNA. The milimolar quantities of [Co(phen) 3 3+ ], which associates reversibly with immobilized calf thymus DNA was detected by using dsDNA‐modified carbon paste electrode (dsDNA‐modified CPE), ssDNA‐modified carbon paste electrode (ssDNA‐modified CPE) and bare carbon paste electrode (bare CPE), voltammetrically and the decreased peak currents were observed, respectively. The extend of hybridization between the complementary sequences is determined by the enhancement of the voltammetric peak of the [Co(phen) 3 3+ ] indicator. Numerous factors affecting the DNA immobilization and indicator were investigated. Experiments were also performed at various salt concentrations and the optimum salt concentration was determined. The difference between the peak currents of denaturated calf thymus DNA (ssDNA)‐modified CPE and dsDNA‐modified CPE was also observed. These results demonstrated the use of the electroactive hybridization indicator, [Co(phen) 3 3+ ] for DNA biosensors.