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Reagentless Sensor Integrating Electrodes, Photodetector, and Immobilized Co‐Substrate for Electrochemiluminescence‐Based Assays
Author(s) -
Michel Philippe E.,
van der Wal Peter D.,
Fiaccabrino Giovanni C.,
de Rooij Nico F.,
KoudelkaHep Milena
Publication year - 1999
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/(sici)1521-4109(199912)11:18<1361::aid-elan1361>3.0.co;2-c
Subject(s) - electrochemiluminescence , detection limit , materials science , photodetector , substrate (aquarium) , electrode , biosensor , transducer , matrix (chemical analysis) , chromatography , analytical chemistry (journal) , optoelectronics , chemistry , nanotechnology , composite material , oceanography , physics , quantum mechanics , geology
A reagentless and regenerable electrochemiluminescence sensor has been developed by immobilizing the Rubpy 3 2+ complex at the surface of a miniaturized sensor combining the electrode transducer and the photodetector on the same silicon chip. The immobilization was performed following a two‐step procedure. The complex was first incorporated in a sol‐gel matrix which was ground to a powder. The microparticles thus obtained were then entrapped in a polyhydroxyethyl methacrylate membrane. The sensor was characterized by performing codeine assays with standard and pharmaceutical samples. The detection limit for codeine was 20 μM and the sensitivity of the sensor represented 20 % of the value obtained when the cosubstrate was supplied in solution. The self‐containment working time was determined to be 7 days of reproducible measurements.