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Amperometric Glucose Enzyme Sensor Prepared by Immobilizing Glucose Oxidase on CuPtCl 6 Chemically Modified Electrode
Author(s) -
Pei Jianhong,
Li Xiaoyuan
Publication year - 1999
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/(sici)1521-4109(199911)11:17<1266::aid-elan1266>3.0.co;2-r
Subject(s) - glucose oxidase , amperometry , glutaraldehyde , electrode , immobilized enzyme , biosensor , chemistry , bovine serum albumin , detection limit , chromatography , nuclear chemistry , inorganic chemistry , enzyme , electrochemistry , biochemistry
A simple, sensitive, stable and low‐cost glucose sensor with a fast response that operates under aerobic solution conditions is described. The electrode consisted of a CuPtCl 6 modified film deposited on the surface of the glassy carbon electrode onto which the glucose oxidase was immobilized via cross‐linking of glutaraldehyde in the presence of bovine serum albumin (BSA). The electropolymerized CuPtCl 6 film was prepared by continuously cyclically scanning between 700 mV and –800 mV (vs. SCE) in a solution containing CuCl 2 and K 2 PtCl 6 . The preparation and the performance of the enzyme electrode have been discussed. The effects of enzyme loading, pH, and the temperature have been studied. The experiments on interference and specificity have also been demonstrated. The enzyme electrode exhibits an excellent biocatalytic activity and stability. The enzyme electrode provides a linear response to glucose over a concentration range of 2×10 –6 M to 1×10 –3 M with a detection limit of 1×10 –6 M.