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Electrochemical Studies of the Intercalation of Ethidium Bromide to DNA
Author(s) -
Tang TzyhChyang,
Huang HsuanJung
Publication year - 1999
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/(sici)1521-4109(199911)11:16<1185::aid-elan1185>3.0.co;2-#
Subject(s) - ethidium bromide , intercalation (chemistry) , electrochemistry , dna , chemistry , bromide , inorganic chemistry , combinatorial chemistry , electrode , biochemistry
Ethidium bromide in a concentration range of 5.7 nM to 0.7 µM was determined amperometrically with an electrochemically activated glassy carbon electrode. From the relationship derived for the intercalation of EB to DNA and the equilibrated concentration of free ethidium bromide determined, the binding constant of the EB‐DNA intercalation was determined. The binding constants found agree very well with those reported in the literature. The linear relationship found for EB‐DNA intercalation was developed as a new procedure for DNA analysis. With the developed method, a detection limit of 0.4 µM and a linear dynamic range of 0.4 to 107 µM for dsDNA analysis can be obtained optimistically.