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A Study of the Electrochemistry of Carnosine Based on the Coupling Reaction with Diazo‐ p ‐aminoacetophenone
Author(s) -
Wu Xiao Ping,
Duan Jian Ping,
Chen Hong Qing,
Chen Guo Nan
Publication year - 1999
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/(sici)1521-4109(199907)11:9<641::aid-elan641>3.0.co;2-a
Subject(s) - carnosine , chemistry , electrochemistry , cyclic voltammetry , redox , diazo , dropping mercury electrode , inorganic chemistry , mercury (programming language) , detection limit , electrode , organic chemistry , chromatography , computer science , programming language , biochemistry
Carnosine could be determined in the range between 1.0×10 –7 and 5.5×10 –6 M by differential pulse adsorptive stripping voltammetry (DPASV) on a static mercury electrode after derivatization with diazo‐ p ‐aminoacetophenone (DPAAP) at pH 9.6–9.7. The detection limit was 6.8×10 –8 M, the relative standard deviation ( n =10) for 5.0×10 –6 M of carnosine was 1.8 %. The coupling reaction and electrochemical reaction mechanism of azo‐carnosine were investigated by structure analysis, UV spectroscopic, cyclic voltammetry, DPASV and electrocapillary at mercury electrodes. A reversible two‐electron via two‐proton redox and adsorption‐controlled process occurred in four consecutive steps with an intermediate at the mercury electrode.