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HPLC Analysis of Carbohydrates with Electrochemical Detection at a Poly‐1‐naphthylamine/Copper Modified Electrode
Author(s) -
D'Eramo Fabiana,
Marioli Juan M.,
Arévalo Alejandro A.,
Sereno Leónides E.
Publication year - 1999
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/(sici)1521-4109(199906)11:7<481::aid-elan481>3.0.co;2-7
Subject(s) - copper , amperometry , electrode , quinhydrone electrode , electrochemistry , glassy carbon , working electrode , inorganic chemistry , palladium hydrogen electrode , materials science , chemistry , cyclic voltammetry , organic chemistry
Carbohydrates are detected by constant potential amperometric detection at a modified electrode consisting of poly‐1‐naphthylamine coated glassy carbon with copper ions incorporated into the polymeric matrix. The polymer is electrodeposited onto the glassy carbon surface by cyclic voltammetric experiments, and copper ions incorporated by immersion of the modified electrode in a 0.10 M cupric ions solution. The amount of copper incorporated into the polymeric matrix is regulated by controlling the immersion time. Cyclic voltammetric experiments showed that glucose was oxidized at the modified electrode surface. The catalytic current for glucose oxidation was dependent on the amount of copper incorporated. Flow injection experiments allowed the determination of the working electrode potential at which maximum signal‐to‐noise ratios were obtained. The electrode was successfully used in the high performance liquid chromatographic analysis of various carbohydrates.