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Immobilization of Lactate Dehydrogenase on Electrochemically Prepared Polyaniline Films
Author(s) -
Gerard Manju,
Ramanathan Kumaran,
Chaubey Asha,
Malhotra B. D.
Publication year - 1999
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/(sici)1521-4109(199905)11:6<450::aid-elan450>3.0.co;2-r
Subject(s) - polyaniline , lactate dehydrogenase , amperometry , electrode , conductive polymer , l lactate dehydrogenase , dehydrogenase , chemistry , electrochemistry , immobilized enzyme , polymer , materials science , enzyme , biochemistry , organic chemistry , polymerization
Immobilization of lactate dehydrogenase (LDH) has been carried out on electrochemically prepared polyaniline (PANI) films. The results of the photometric and amperometric response measurements conducted on such LDH/PANI electrodes show the response time as 90 s and shelf‐life as about two weeks at 4–10 °C indicating that these conducting polymer‐enzyme electrodes can be used as a pyruvate sensor.