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Small‐Volume Electrochemical Detection of Trace Nucleic Acids Using a Vibrating Electrode System
Author(s) -
Wang Joseph,
Sahlin Eskil,
Paz Jose Luis Lopez
Publication year - 1999
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/(sici)1521-4109(199905)11:5<380::aid-elan380>3.0.co;2-k
Subject(s) - nucleic acid , volume (thermodynamics) , electrode , chemistry , stripping (fiber) , analytical chemistry (journal) , detection limit , evaporation , chromatography , materials science , biochemistry , physics , thermodynamics , composite material
Nanogram and picogram amounts of nucleic acids can be readily detected by combining adsorptive‐stripping chronopotentiometry with a small‐volume vibrational‐electrode system. Such coupling of microliter sample volumes, an effective vibrational mass transport, and sophisticated baseline fitting results in remarkably low (sub‐fmol) mass detection limits for DNA and RNA. These improvements, along with the challenges inherent to the small‐volume detection (e.g., depletion, evaporation), and prospects to small‐volume label‐free hybridization assays, are discussed.