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Benzoquinone‐Mediated Biosensor for Amperometric Determination of D ‐Lysine
Author(s) -
Murthy A. Surya N,
Sharma Jyotsna
Publication year - 1999
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/(sici)1521-4109(199903)11:3<188::aid-elan188>3.0.co;2-4
Subject(s) - amperometry , biosensor , lysine , chemistry , benzoquinone , electron transfer , amino acid , chromatography , oxidase test , glassy carbon , electrode , enzyme , analytical chemistry (journal) , biochemistry , photochemistry , electrochemistry , cyclic voltammetry
An amperometric enzyme sensor for the analysis of D ‐lysine is described. The sensor uses benzoquinone as mediator for electron transfer between immobilized D ‐amino acid oxidase and a glassy carbon electrode. A linear current response, proportional to D ‐lysine concentration in the range 1–10mM, was observed. The influence of oxygen, pH, and operating potential on the sensor performance were examined.