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A New HPLC Method for the Determination of Polyphenols in Wines Based on the Use of Less Aggressive Eluents and a Coupled Revelation System
Author(s) -
Brenna Oreste,
Buratti Susanna,
Cosio Maria Stella,
Mannino Saverio
Publication year - 1998
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/(sici)1521-4109(199811)10:17<1204::aid-elan1204>3.0.co;2-i
Subject(s) - analyte , chromatography , chemistry , high performance liquid chromatography , polyphenol , selectivity , resolution (logic) , analytical chemistry (journal) , organic chemistry , computer science , artificial intelligence , antioxidant , catalysis
A new reverse‐phase HPLC method that uses ‘nonaggressive’ eluents and a coupled revelation system based on diode‐array and dual electrode electrochemical detection is described. This method is suitable for direct measurement of polyphenols in wines and offers distinct advantages in terms of selectivity and sensitivity when compared with alternative HPLC methods, based on UV‐vis or PDA detection, since multiple features of the analytes can be checked during sample analysis. Good peak resolution was obtained following direct injection of a 20 μL sample, and more than 20 different analytes were identified by spectral comparison with known standards and by their electrochemical behavior at potentials of +0.4 and +0.8 V (vs Ag/AgCl).