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Natural Polymer‐Based Sulfite Biosensor
Author(s) -
Ng LooTeck,
Yuan Yong J.,
Zhao Huijun
Publication year - 1998
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/(sici)1521-4109(199811)10:16<1119::aid-elan1119>3.0.co;2-u
Subject(s) - chitosan , methacrylate , biosensor , polymer , sulfite oxidase , polymer chemistry , immobilized enzyme , polymerization , sulfite , chemistry , covalent bond , chemical engineering , materials science , combinatorial chemistry , organic chemistry , nanotechnology , enzyme , engineering
As a natural product, chitosan has the inherent properties of being biodegradable, biocompatible and nontoxic. These properties render it an ideal matrix in enzyme immobilization for clinical analysis. In this work, the feasibility of electrochemical biosensor fabrication using chitosan has been demonstrated. As a test case, the enzyme, sulfite oxidase (SOD), was covalently immobilized onto the matrix of chitosan‐poly(hydroxyethyl methacrylate) (chitosan‐HEMA), a natural/synthetic polymer hybrid material obtainable via a UV curing process. An electron transfer mediator, p ‐benzoquinone was coupled onto the polymer network for the activation of chitosan‐HEMA copolymer after the photo‐induced polymerization reaction. The biological activity of the immobilized SOD and the electroactivity of the coupled p ‐benzoquinone were examined.