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Protein Detection Using Conducting Polymer Microarrays
Author(s) -
Lu W.,
Nguyen T. A.,
Wallace Gordon G.
Publication year - 1998
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/(sici)1521-4109(199811)10:16<1101::aid-elan1101>3.0.co;2-6
Subject(s) - polymer , principal component analysis , microelectrode , materials science , chromatography , analytical chemistry (journal) , biological system , chemistry , electrode , computer science , artificial intelligence , organic chemistry , biology
An array of conducting polymer coated microelectrodes was employed as an amperometric detector to analyze a range of proteins. Using conducting polymer coatings with different counterions incorporated, varying selectivity series have been obtained. Protein identification and quantification were performed using chemometric techniques such as principal component analysis (PCA), soft independent modelling of class analogy (SIMCA), ordinary least square (OLS) and partial least square (PLS). Using four different polymers, response patterns were obtained and classification of six proteins was achieved. Individual proteins in a two component mixture were quantitatively analyzed with acceptable accuracy.