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Immunoelectrochemical Assay in Combination with Homogeneous Enzyme‐Labeled Antibody Conjugation for Rapid Detection of Salmonella
Author(s) -
Yang Zhongping,
Li Yanbin,
Balagtas Christopher,
Slavik Michael,
Paul David
Publication year - 1998
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/(sici)1521-4109(199810)10:13<913::aid-elan913>3.0.co;2-0
Subject(s) - salmonella , detection limit , chemistry , conjugate , differential pulse voltammetry , chromatography , alkaline phosphatase , phenol , substrate (aquarium) , enzyme , antibody , homogeneous , immunoassay , microbiology and biotechnology , biochemistry , electrode , cyclic voltammetry , bacteria , biology , electrochemistry , organic chemistry , mathematics , mathematical analysis , ecology , genetics , immunology , thermodynamics , physics
An immunoelectrochemical assay in combination with homogeneous enzyme‐labeled antibody conjugation was developed for rapid detection of Salmonella . The assay was performed by mixing alkaline phosphatase linked anti‐ Salmonella (APLAS) with Salmonella in a solution. The Salmonella ‐APLAS conjugate separated by polycarbonate membrane filtration was incubated with a phenyl phosphate substrate to produce phenol. The concentration of Salmonella cells was determined by measuring phenol oxidation peak current using differential pulse voltammetry at a renewable carbon paste electrode. This assay could be completed within two hours with a detection limit of 5 × 10 3 cells/mL. A linear response was found for Salmonella between 5 × 10 3 and 1 × 10 6 cells/mL.