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Electrogeneration of Biotinylated Functionalized Polypyrroles for the Simple Immobilization of Enzymes
Author(s) -
Cosnier Serge,
Galland Bruno,
Gondran Chantal,
Le Pellec Arielle
Publication year - 1998
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/(sici)1521-4109(199809)10:12<808::aid-elan808>3.0.co;2-k
Subject(s) - biotinylation , avidin , glucose oxidase , copolymer , chemistry , biosensor , pyrrole , amperometry , biotin , polymer chemistry , adsorption , surface modification , combinatorial chemistry , polymerization , immobilized enzyme , polymer , organic chemistry , electrode , enzyme , biochemistry , electrochemistry
A new biotin derivative functionalized by a pyrrole group has been synthesized. This functionalization has allowed the preparation of the first example of a poly(pyrrole‐biotin) film by oxidative polymerization. In addition, the successful copolymerization of a polypyridinyl complex of ruthenium(II) containing pyrrole groups with the biotin derivative provided redox copolymers exhibiting specific adsorption sites. The resulting copolymer electrodes were applied to the immobilization of a biotinylated glucose oxidase through an avidin bridge. An avidin‐conjugated alkaline phosphatase was also immobilized by direct specific adsorption onto the biotinylated polymer. The amperometric response of the resulting biosensors to glucose and phenylphosphate has been studied.