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A Reagentless Biosensor for the Amperometric Determination of NADH
Author(s) -
Cosnier Serge,
Décout JeanLuc,
Fontecave Marc,
Frier Christelle,
Innocent Christophe
Publication year - 1998
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/(sici)1521-4109(199807)10:8<521::aid-elan521>3.0.co;2-r
Subject(s) - amperometry , biosensor , flavin group , chemistry , pyrrole , flavin adenine dinucleotide , detection limit , riboflavin , combinatorial chemistry , chromatography , enzyme , electrode , organic chemistry , cofactor , electrochemistry , biochemistry
A flavin analog ( 1 ) in which the isoalloxazine ring of riboflavin is linked to a pyrrole group has been synthesized and electrochemically characterized. The enzyme flavin reductase (Fre) was successfully immobilized by copolymerization of compound 1 and the amphiphilic pyrrole ammonium derivative 2 . The amperometric response of the resulting poly( 1 ‐ 2 )‐Fre electrode to NADH was based on the oxidation of the enzymically reduced flavin analog 1 at −0.1 V (vs. SCE). The sensitivity and detection limit of the biosensor for NADH are 13.7 mA M −1 cm −2 and 0.5 μM, respectively. The new biosensor is NADH‐specific since no amperometric response was observed for repeated additions of NADPH.