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A Biosensor System for Chlorophenols Using Chloroperoxidase and a Glucose Oxidase Based Amperometric Electrode
Author(s) -
Saby Coralie,
Luong John H. T.
Publication year - 1998
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/(sici)1521-4109(199801)10:1<7::aid-elan7>3.0.co;2-j
Subject(s) - chemistry , glucose oxidase , hydrogen peroxide , pentachlorophenol , cyclic voltammetry , amperometry , chlorophenol , biosensor , nuclear chemistry , inorganic chemistry , electrode , electrochemistry , organic chemistry , biochemistry , phenol
A combined electrocatalytic approach using chloroperoxidase and a glucose oxidase electrode has been developed for the measurement of chlorophenol (CP) congeners. Chloroperoxidase possessed a capability to generate a significant or detectable amount of electroactive species from the oxidation of 4‐CP, 2,3‐dichlorophenol (DCP), 2,4‐DCP, 2,5‐DCP, 3,4‐DCP, 3,5‐DCP, 2,3,4‐trichlorophenol (TCP), 2,3,5‐TCP, 2,3,6‐TCP, 2,4,5‐TCP, 2,4,6‐TCP, and 2,3,4,6‐tetraCP. The optimal oxidation conditions were established as 33 μM chlorophenol, 33 U/mL chloroperoxidase and 1.33 mM hydrogen peroxide for 15 min at ambient temperature. The chlorophenol oxidation products have also been characterized using UV‐vis spectrophotometry and cyclic voltammetry (CV). Together with glucose oxidase immobilized on a working glassy carbon electrode (+0.45 V vs. Ag/AgCl), chlorinated oxidation products have been demonstrated as efficient mediators in a glucose oxidase/glucose system. However, the oxidation products from the enzymatic reaction with 2‐CP, 3‐CP, 2,6‐DCP, 3,4,5‐TCP, 2,3,5,6‐tetraCP and pentachlorophenol were not electroactive or capable of recycling reduced glucose oxidase to its reactive state.