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Biotin‐Modified Surfaces by Electrochemical Polymerizationof Biotinyl‐Tyramide
Author(s) -
Yang SeungTae,
Witkowski Allan,
Hutchins Richard S.,
Scott Donna L.,
Bachas Leonidas G.
Publication year - 1998
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/(sici)1521-4109(199801)10:1<58::aid-elan58>3.0.co;2-z
Subject(s) - avidin , chemistry , biotin , indium tin oxide , electrochemistry , glassy carbon , cyclic voltammetry , electrode , conjugate , nuclear chemistry , biotinylation , inorganic chemistry , biochemistry , mathematical analysis , mathematics
Poly(biotinyl‐tyramide) films were created by the electrochemical oxidation of biotinyl‐tyramide. Electropolymerization in basic solutions such as NaOH/methanol or aqueous NaOH led to adherent poly(biotinyl) films on both glassy‐carbon (GC) and indium(tin) oxide (ITO) electrodes. Cyclic voltammetry revealed that the resulting poly(biotinyl‐tyramide) films passivated the electrode surface. Studies using an avidin‐β′‐galactosidase conjugate showed that the films provide biotin sites capable of selectively binding avidin.