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Tissue reactions following total hip arthroplasty – a Raman microscopic study on dogs
Author(s) -
Pingsmann A.,
Dippel B.,
Müller R. T.
Publication year - 1999
Publication title -
materialwissenschaft und werkstofftechnik
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.285
H-Index - 38
eISSN - 1521-4052
pISSN - 0933-5137
DOI - 10.1002/(sici)1521-4052(199912)30:12<801::aid-mawe801>3.0.co;2-z
Subject(s) - implant , chemistry , raman spectroscopy , raman microscope , bone tissue , mineralization (soil science) , nuclear medicine , biomedical engineering , surgery , medicine , physics , organic chemistry , nitrogen , optics , raman scattering
A quantitative determination of the mineralization of bone tissue and of the hydroxyapatite (HA) content of HA coatings following total hip arthroplasties in dogs was performed using an optimised FT Raman microscope (inverted microscope, high throughput of radiation) that allows minimal sample preparation without fluorescence. The lateral resolution reached down to 10 μm. A similar cross‐sectional course of the HA content – reaching from the implant body to the ongrown bone ‐ was found in all cases. In the immediate vicinity of the prosthesis a large HA content could be observed that decreased to a minimum towards the periphery of the coating and increased at the site of the ongrown bone. For the interface between bone and HA coating a transitional zone was observed at a lateral distance of 30–40 μm to the implant. Bone ingrown into the gap between detached HA coating and implant had a lower HA content than bone ongrown onto the coating peripherally. Thus, Raman microscopy can determine the relative age of bone tissue at different sites in the course of implant integration.