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Cloning and expression of a plasmid‐linked pediocin determinant trait of Pediococcus acidilactici F
Author(s) -
Osmanağaoğlu Özlem,
Beyatli Yavuz,
Gündüz Ufuk
Publication year - 2000
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/(sici)1521-4028(200002)40:1<41::aid-jobm41>3.0.co;2-#
Subject(s) - pediococcus acidilactici , pediococcus , plasmid , cloning (programming) , biology , genetics , gene , bacteria , lactobacillus , computer science , lactic acid , lactobacillus plantarum , programming language
Plasmid DNA from Pediococcus acidilactici F was prepared by lysozyme‐mutanolysin method and purified by cesium chloride‐ethidium bromide (CsCl‐EtBr) density gradient ultracentrifugation. Agarose gel electrophoresis of plasmid DNA and plasmid‐curing experiments suggested that bacteriocin activity was harboured on a small plasmid of approximately 9.1 kb (kilobasepair) in Pediococcus acidilactici F. Plasmid encoding bacteriocin production in P. acidilactici F was examined for restriction enzyme cleavage patterns and its map has been constructed. An Escherichia coli strain transformed with the recombinant plasmid, pQE322, produced and, most probably, secreted pediocin F.