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Enzymes of carbohydrate metabolism in root‐nodule bacteria during growth on acetate
Author(s) -
Mandal Narayan Chandra,
Chakrabartty Pran K.
Publication year - 1999
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/(sici)1521-4028(199909)39:4<253::aid-jobm253>3.0.co;2-c
Subject(s) - glyoxylate cycle , citric acid cycle , biochemistry , pentose phosphate pathway , enzyme , dehydrogenase , bacteria , metabolism , carbohydrate metabolism , biology , glycolysis , tricarboxylic acid , gluconeogenesis , metabolic pathway , rhizobium , genetics , gene
The key enzymes of the Embden ‐ Meyerhof ‐ Parnas (EMP), Entner ‐ Doudoroff (ED) and pentose phosphate (PP) pathways as well as those of the tricarboxylic acid (TCA) cycle and gluconeogenesis were assayed in the cells of Rhizobium and Bradyrhizobium grown on acetate. Except for glyceraldehyde‐3‐phosphate dehydrogenase, the activities of all the enzymes of the EMP, ED and PP pathways were found to be low in acetate grown cells in the present study as compared to those of glucose grown cells reported by us earlier ( Mandal and Chakrabartty 1993) indicating a probable repression of the enzymes. The enzymes of the tricarboxylic acid cycle were detected in high levels in the acetate grown cells of the root‐nodule bacteria. The operation of the glyoxylate pathway for acetate metabolism in the root‐nodule bacteria were reported previously by us ( Mandal and Chakrabartty 1992). The evidences taken together point to the simultaneous operation of both the TCA cycle and the glyoxylate cycle for acetate metabolism in these bacteria.