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Transformation of a flocculating Saccharomyces cerevisiae using lithium acetate and pYAC4
Author(s) -
Venâncio Armando,
Domingues Lucília,
Lima Nelson
Publication year - 1999
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/(sici)1521-4028(199903)39:1<37::aid-jobm37>3.0.co;2-5
Subject(s) - transformation (genetics) , transformation efficiency , ura3 , saccharomyces cerevisiae , yeast , plasmid , chemistry , lysis , flocculation , shuttle vector , spheroplast , dna , chromatography , biochemistry , recombinant dna , microbiology and biotechnology , biology , gene , vector (molecular biology) , organic chemistry , agrobacterium , escherichia coli
A flocculating yeast Saccharomyces cerevisiae ura3 was transformed by the method based on treatment of intact cells with lithium acetate plus single‐stranded carrier DNA using the shuttle vector pYAC4. The transformation efficiency was above 10 3 transformants per microgram of plasmid DNA which is similar to other described yeast transformation systems. Under selective pressure, the transformed cells were stable and maintained the flocculation ability. Thus, this simple transformation system can be used for gene expression studies in flocculating yeasts, overcoming disadvantages of conventional methods such as the spheroplast one.