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Serum sensitivity and cell surface hydrophobicity of Klebsiella pneumoniae treated with gentamicin, tobramycin and amikacin
Author(s) -
Hoštacká Anna
Publication year - 1998
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/(sici)1521-4028(199811)38:5/6<383::aid-jobm383>3.0.co;2-j
Subject(s) - tobramycin , amikacin , gentamicin , klebsiella pneumoniae , antibiotics , microbiology and biotechnology , aminoglycoside , bacteria , in vivo , chemistry , biology , escherichia coli , biochemistry , genetics , gene
A suppression of bacterial growth of Klebsiella pneumoniae after 30 min treatment with gentamicin, tobramycin and amikacin at suprainhibitory concentrations was found (postantibiotic effect, PAE). The antibiotics at a concentration of 2 × MIC induced PAEs in the range of 0.5–1.7 h, PAEs manifested by aminoglycosides at a concentration of 4 × MIC varied between 0.8–3.3 h. Susceptibility of exposed bacteria to serum bactericidal activity was efficiently enhanced compared with controls (without antibiotics). A survival of treated bacteria was between 12.7–36.6% (2 × MIC) or 5.0–8.3% (4 × MIC). The percentage of viable nontreated bacteria moved between 70.2–83.0% at these conditions. Surface hydrophobicity of bacteria exposed to aminoglycosides was only slightly increased. The results indicate that exposure of K. pneumoniae to a suprainhibitory concentrations of aminoglycosides in vitro enhanced the susceptibility of this strain to human serum bactericidal activity. Whether this phenomenon occurs in vivo , remains to be determined.