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Detection of N 6 ‐methyladenine in GATC sequences of Selenomonas ruminantium
Author(s) -
Pristas Peter,
Molnarova Veronika,
Javorsky Peter
Publication year - 1998
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/(sici)1521-4028(199809)38:4<283::aid-jobm283>3.0.co;2-4
Subject(s) - biology , methylation , restriction enzyme , dna , dna methylation , genetics , microbiology and biotechnology , gene , gene expression
The presence of N 6 ‐methyladenine in GATC sequences in DNA of Selenomonas ruminantium was investigated using sensitive methylation discriminating isochizomeric restriction enzymes analysis. Methylated adenine was detected in 8 out of 18 tested strains belonging to the subsp. lactilytica of S. ruminantium . No corresponding restriction activity was detected in three tested strains. No GATC methylation was detected in 3 analysed S. ruminantium subsp. ruminantium strains.