Premium
Effect of osmotic stress on glucose oxidase production and secretion by Aspergillus niger
Author(s) -
Fiedurek J.
Publication year - 1998
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/(sici)1521-4028(199805)38:2<107::aid-jobm107>3.0.co;2-1
Subject(s) - aspergillus niger , glucose oxidase , mycelium , catalase , osmotic shock , osmotic concentration , chemistry , enzyme , biochemistry , food science , periplasmic space , biology , botany , gene , escherichia coli
Abstract A mycelium of Aspergillus niger was cultured under various osmotic stresses. When fungus was cultured under osmotic stress, total catalase activity increased with increasing concentration of NaCl, up 0.4 M , but glucose oxidase under this condition significantly decreased. Mycelial growth was repressed with increased media osmolarity. To release periplasmic glucose oxidase 72‐h old mycelium was suspended on a concentrated solution of NaCl (0.4—2.8 M /l). The highest yield of GOD activity was obtained at a NaCl concentration of 1.2 M at pH 6.0, which improved the activity of this enzyme by about 2.1‐fold in comparison with the control medium without this depressor.