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New shuttle vectors for Rhodococcus sp. R312 (formerly Brevibacterium sp. R312), a nitrile hydratase producing strain
Author(s) -
Duran Robert
Publication year - 1998
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/(sici)1521-4028(199805)38:2<101::aid-jobm101>3.0.co;2-p
Subject(s) - shuttle vector , kanamycin , brevibacterium , replicon , plasmid , transformation (genetics) , nitrile hydratase , chloramphenicol , biology , rhodococcus , microbiology and biotechnology , strain (injury) , bacteria , gene , genetics , vector (molecular biology) , recombinant dna , biochemistry , antibiotics , enzyme , microorganism , anatomy
Two shuttle vectors named pRC52 (10.7 Kb) and pRK52 (12.2 Kb) carrying chloramphenicol (Cm) and chloramphenicol plus kanamycin (Km) resistance genes, respectively, were constructed by fusion of a cryptic plasmid pBL13869 (replicon pBL1, 5.8 Kb) from Brevibacterium lactofermentum ATCC13869 with pBR328 E. coli plasmid. Transformation of Rhodococcus sp. R312 (formerly Brevibacterium sp. R312) protoplasts was realised with an efficiency of 28 transformants per μg of DNA.