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Characterization of polyisoprene by temperature gradient interaction chromatography
Author(s) -
Lee Wonmok,
Lee Hee Cheong,
Park Taiho,
Chang Taihyun,
Chae Kyu Ho
Publication year - 2000
Publication title -
macromolecular chemistry and physics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.57
H-Index - 112
eISSN - 1521-3935
pISSN - 1022-1352
DOI - 10.1002/(sici)1521-3935(20000201)201:3<320::aid-macp320>3.0.co;2-4
Subject(s) - fractionation , polystyrene , chemistry , column chromatography , resolution (logic) , chromatography , size exclusion chromatography , phase (matter) , gel permeation chromatography , elution , molar mass distribution , polymer chemistry , analytical chemistry (journal) , organic chemistry , polymer , artificial intelligence , computer science , enzyme
Temperature gradient interaction chromatography (TGIC) analysis of polyisoprene (PI) is reported. PI can be fractionated in terms of molecular weight with high resolution by TGIC using a C18 bonded silica column as the stationary phase and a mixture of CH 2 Cl 2 /CH 3 CN (80/20, v/v) as the mobile phase. The temperature was increased during the elution from 5°C to 34°C to optimize the fractionation of high molecular weight PI. The relatively poor resolution in the low molecular weight range was improved by changing the eluent composition. For example, all the oligomeric species from the unimer to the 29mer of a low molecular weight PI (average mol. wt.: 900) were fully resolved with the mixed eluent of CH 2 Cl 2 /CH 3 CN (50/50, v/v) and a linear temperature program from 5°C to 40°C. In addition, simultaneous size exclusion chromatography and TGIC fractionation of PI/polystyrene, PI/poly(methylmethacrylate) mixtures are also reported.