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Self‐aggregate nanoparticles of cholesteryl and galactoside groups‐substituted pullulan and their specific binding to galactose specific lectin, RCA 120
Author(s) -
Taniguchi Ikuo,
Akiyoshi Kazunari,
Sunamoto Junzo
Publication year - 1999
Publication title -
macromolecular chemistry and physics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.57
H-Index - 112
eISSN - 1521-3935
pISSN - 1022-1352
DOI - 10.1002/(sici)1521-3935(19990601)200:6<1554::aid-macp1554>3.0.co;2-v
Subject(s) - galactosides , chemistry , pullulan , lectin , galactose , hydrodynamic radius , nanoparticle , polymer chemistry , dynamic light scattering , moiety , dendrimer , galactoside , polysaccharide , stereochemistry , polymer , organic chemistry , biochemistry , nanotechnology , materials science , copolymer , enzyme
The hydrophobized polysaccharide cholesterol‐bearing pullulan (CHP) forms hydrogel nanoparticles upon self‐aggregation in water. To endow cell specificity to nanoparticles of CHP, galactosides were additionally substituted to CHP with various spacer lengths and degrees of substitution. The resulting CHP derivatives also form monodisperse nanoparticles by self‐aggregation in water. All self‐aggregate nanoparticles strongly bind to the β ‐ D ‐galactose‐specific lectin, RCA 120 ( Ricinus communis agglutinin ). The affinity of substituted galactosides to the lectin was quantitatively studied using a fluorescence polarization technique by competitive binding with 4‐methylumbelliferyl β ‐ D ‐galactopyranoside (MUG). This affinity strongly depends on its chemical structure, degree of substitution, and spacer length. The binding constant per galactose moiety drastically increased by substitution to the polymer backbone.