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Preparation of organized protein multilayers
Author(s) -
Brynda Eduard,
Houska Milan
Publication year - 1998
Publication title -
macromolecular rapid communications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.348
H-Index - 154
eISSN - 1521-3927
pISSN - 1022-1336
DOI - 10.1002/(sici)1521-3927(19980401)19:4<173::aid-marc173>3.0.co;2-s
Subject(s) - isoelectric point , covalent bond , adsorption , chemistry , buffer (optical fiber) , chemical engineering , protein adsorption , self assembly , polymer chemistry , materials science , chromatography , nanotechnology , organic chemistry , computer science , telecommunications , engineering , enzyme
Abstract A novel technique of preparation of multilayer assemblies consisting of a controlled number of molecular layers of various proteins arranged according to a predetermined architecture is described. The multilayers were formed by consecutive adsorption of polyanions and positively charged proteins below their isoelectric point and covalently crosslinked via protein amino groups. Polyanions were washed out from the crosslinked protein assembly by rinsing with buffer in which the net charge of the proteins was inverted.