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MALDI‐MS Characterization of Maltooligo/polysaccharides From Debranched Starch Amylopectin of Corn and Barley
Author(s) -
Wang Jian,
Jiang Gaosong,
Vasanthan Thava,
Sporns Peter
Publication year - 1999
Publication title -
starch ‐ stärke
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.62
H-Index - 82
eISSN - 1521-379X
pISSN - 0038-9056
DOI - 10.1002/(sici)1521-379x(199907)51:7<243::aid-star243>3.0.co;2-b
Subject(s) - amylopectin , waxy corn , starch , amylose , chemistry , polysaccharide , food science , resistant starch , chromatography , biochemistry
Aprotocol was developed for molecular characterization of debranched starch amylopectin using Matrix‐assisted laser desorption/ionizationmass spectrometry (MALDI‐MS). Starch from waxy corn was used in the protocol development. Various factors influencing the protocol were investigated and discussed. Using the developed protocols, starch amylopectin from barley grains (waxy‐type Candle and regular‐type Phoenix) were also characterized. The study suggested that MALDI‐MS is suitable to determine the molecular size profile of debranched starch (maltooligo‐ and polysaccharides) faster and with greater accuracy than any present analytical methodology.