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Structural and Physicochemical Properties of Endosperm Starches Possessing Different Alleles at the Amylose‐extender and Waxy Locus in Maize ( Zea mays L.)
Author(s) -
Fuwa Hidetsugu,
Inouchi Naoyoshi,
Glover David V.,
Fujita Shuzo,
Sugihara Miyuki,
Yoshioka Sakiko,
Yamada Keiko,
Sugimoto Yoshimi
Publication year - 1999
Publication title -
starch ‐ stärke
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.62
H-Index - 82
eISSN - 1521-379X
pISSN - 0038-9056
DOI - 10.1002/(sici)1521-379x(199905)51:5<147::aid-star147>3.0.co;2-7
Subject(s) - endosperm , amylose , amylopectin , starch , chemistry , maize starch , inbred strain , food science , biochemistry , gene
The structure and some physicochemical properties of endosperm starches from seven amylose‐extender ( ae ) and two waxy ( wx ) alleles of maize ( Zea mays L.) were studied. Starches prepared from mature kernels of six ae mutants, Oh43 inbred line ae (standard ae ), ae ‐RWB‐2 and ae ‐RWB‐3, and W23 × L317 hybrid line ae ‐PP, ae ‐Bol 561 and ae ‐emll, were uniquely ae type, as was concluded from B type X‐ray diffractograms; high gelatinization temperatures determined by differential scanning calorimetry (DSC); with poor starch‐granule digestibility to amylase; high amylose (37—45 %) and high intermediate fraction (13—18 %) contents; and low ratios (1.0—1.2) of long α‐1,4‐chains to short α‐1,4‐chains of amylopectin determined by gel permeation chromatography (GPC) of isoamylase‐debranched starches. The results also indicated that different ae alleles had different effects on the amylose content of endosperm starches. Mature kernels of Oh43 ae ‐RWB‐1 mutant showed tarnished and translucent phenotype characteristics of the ae genotype but contained endosperm starch with 21—22 % of amylose which was lower than that of the Oh43 normal counterpart. Both wx ‐B and wx ‐C (standard wx ) genes have similar effect on structure and physicochemical properties of waxy starches of A632, B37, C105, Oh43, and W64A inbred lines.