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Improved Performance of Amberlite IRA‐904 Immobilized Glucoamylase
Author(s) -
Balakumar Santhirasegarampillai,
Arasaratnam Vasanthy,
Balasubramaniam Kandiah
Publication year - 1998
Publication title -
starch ‐ stärke
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.62
H-Index - 82
eISSN - 1521-379X
pISSN - 0038-9056
DOI - 10.1002/(sici)1521-379x(199803)50:2/3<93::aid-star93>3.0.co;2-8
Subject(s) - amberlite , chemistry , elution , chromatography , substrate (aquarium) , yield (engineering) , nuclear chemistry , adsorption , materials science , organic chemistry , biology , ecology , metallurgy
When the glucoamylase protein immobilized to Amberlite IRA‐904 was estimated by direct ( Kjeldhal ), indirect (measuring the protein left in the supernatant) and elution methods, direct and indirect methods gave almost same results while the elution method gave a protein concentration of only 68.9% of that obtained with the Kjeldhal method. When the activity of the soluble and immobilized glucoamylase were compared with different substrates, the activity yield increased with decreasing molecular size of the substrate. However when the immobilized glucoamylase containing Amberlite IRA‐904 was ground, it showed increase in activity with increasing molecular weight of the substrates.