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A Novel Solid‐Phase Assembly for Identifying Potent and Selective RNA Ligands
Author(s) -
Luedtke Nathan W.,
Tor Yitzhak
Publication year - 2000
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/(sici)1521-3773(20000515)39:10<1788::aid-anie1788>3.0.co;2-6
Subject(s) - biotinylation , rna , streptavidin , chemistry , combinatorial chemistry , ternary complex , fluorescence , peptide , ternary operation , solid phase synthesis , biotin , biophysics , biochemistry , computational biology , biology , computer science , enzyme , physics , quantum mechanics , programming language , gene
A simple, rapid, and quantitative assay that facilitates the discovery and characterization of new RNA binders has been developed. The assembly consists of an ternary complex, in which a fluorescent Rev peptide (Rev is an essential HIV‐1 regulatory protein) binds to its high affinity site on the RRE (Rev‐response element), and this biotinylated RNA, in turn, is immobilized on a solid support through streptavidin.