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Synthesis of the N‐Terminal N ‐Myristoylated and S ‐Palmitoylated Undetrigintapeptide of Endothelial NO‐Synthase
Author(s) -
Machauer Rainer,
Waldmann Herbert
Publication year - 2000
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/(sici)1521-3773(20000417)39:8<1449::aid-anie1449>3.0.co;2-a
Subject(s) - enos , caveolae , chemistry , endothelial nitric oxide synthase , terminal (telecommunication) , membrane , enzyme , palmitoylation , biochemistry , microbiology and biotechnology , computer science , biology , nitric oxide synthase , computer network , cysteine
Endothelial NO synthase (eNOS) is a membrane‐bound protein involved in signaling from the blood stream to the surrounding smooth muscle cells. The synthesis of its N ‐myristolyated and doubly S‐ palmitoylated N‐terminus 1 , which determines the localization of this protein in the plasma membrane and caveolae of endothelial cells, is achieved by a combination of enzyme‐, acid‐, and noble‐metal‐sensitive protecting groups with solid‐phase methods and fragment‐condensation techniques.