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Surface‐Enhanced Resonance Raman Scattering as a Novel Method of DNA Discrimination
Author(s) -
Graham Duncan,
Mallinder Benjamin J.,
Smith W. Ewan
Publication year - 2000
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/(sici)1521-3773(20000317)39:6<1061::aid-anie1061>3.0.co;2-9
Subject(s) - raman scattering , resonance (particle physics) , oligonucleotide , raman spectroscopy , surface (topology) , scattering , dna , materials science , analytical chemistry (journal) , chemistry , molecular physics , optics , chromatography , atomic physics , physics , biochemistry , mathematics , geometry
DNA analysis often requires a separation step; this step is avoided if surface‐enhanced resonance Raman scattering (SERRS) is used. SERRS is shown to differentiate between two labeled oligonucleotide strands, in varying proportions, in a mixture, and at low concentration levels. The method is discriminatory, sensitive, and requires only commonly available equipment.