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How Strong Is the Coordination Bond between a Histidine Tag and Ni – Nitrilotriacetate? An Experiment of Mechanochemistry on Single Molecules
Author(s) -
Conti Matteo,
Falini Giuseppe,
Samorì Bruno
Publication year - 2000
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/(sici)1521-3773(20000103)39:1<215::aid-anie215>3.0.co;2-r
Subject(s) - mechanochemistry , molecule , histidine , chemistry , coordination complex , stereochemistry , nanotechnology , crystallography , materials science , organic chemistry , metal , enzyme
Isolation of a His‐tagged protein by means of a Ni – nitrilotriacetate‐functionalized chromatographic matrix or biosensor was simulated in a single‐molecule experiment, and the mechanochemistry—that is, the interplay between mechanical and chemical forces (shown schematically)—was studied with a scanning force microscope.