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Chemically Modified Antisense Oligonucleotides—Recent Improvements of RNA Binding and Ribonuclease H Recruitment
Author(s) -
Seitz Oliver
Publication year - 1999
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/(sici)1521-3773(19991203)38:23<3466::aid-anie3466>3.0.co;2-x
Subject(s) - oligonucleotide , guanine , rna , nucleobase , nucleotide , cytosine , chemistry , rnase p , ribonuclease , rnase h , biochemistry , dna , stereochemistry , gene
The incorporation of rationally designed sugar or nucleobase modifications into oligonucleotides gives remarkable improvements of the antisense activity. For example, the cytosine analogue 1 recognizes both the Watson–Crick and the Hoogsteen site of a guanine nucleotide 2 , which dramatically enhances the RNA‐binding affinity and selectivity.