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Fluorescent Indicators for Imaging Nitric Oxide Production
Author(s) -
Kojima Hirotatsu,
Urano Yasuteru,
Kikuchi Kazuya,
Higuchi Tsunehiko,
Hirata Yasunobu,
Nagano Tetsuo
Publication year - 1999
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/(sici)1521-3773(19991102)38:21<3209::aid-anie3209>3.0.co;2-6
Subject(s) - fluorescence , intracellular , nitric oxide , schematic , fluorescence microscope , chemistry , yield (engineering) , biophysics , biochemistry , biology , materials science , organic chemistry , optics , physics , electronic engineering , metallurgy , engineering
The membrane‐permeating indicator DAF‐FM DA is transformed by intracellular esterases into the highly water‐soluble dye DAF‐FM, which traps NO produced by NO synthase (NOS) to yield a highly fluorescent triazole compound in cells (see schematic diagram). Monitoring with a fluorescence microscope should allow direct identification of intracellular production and location of NO.