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Specific RNA Dinucleotide Cleavage by a Synthetic Calix[4]arene‐Based Trinuclear Metallo( II )‐phosphodiesterase
Author(s) -
Molenveld Peter,
Engbersen Johan F. J.,
Reinhoudt David N.
Publication year - 1999
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/(sici)1521-3773(19991102)38:21<3189::aid-anie3189>3.0.co;2-x
Subject(s) - chemistry , catalysis , nucleobase , stereochemistry , rna , enzyme , cleavage (geology) , phosphodiesterase , transesterification , substrate (aquarium) , combinatorial chemistry , dna , biochemistry , biology , paleontology , ecology , fracture (geology) , gene
Large rate enhancements and considerable nucleobase specificity in the catalytic transesterification of RNA dinucleotides are observed with the calix[4]arene enzyme mimic 1 ‐Zn 3 (the computer‐generated model of the complex with GpG is shown). The higher activity toward GpG over ApA (factor of 160) originates from favorable substrate binding and cooperative catalytic action of all three Zn II ions. The heterotrinuclear metallo‐phosphoesterase mimic 1 ‐Zn 2 Cu is even more active.