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Enzymatic Regeneration of 3′‐Phosphoadenosine‐5′‐Phosphosulfate Using Aryl Sulfotransferase for the Preparative Enzymatic Synthesis of Sulfated Carbohydrates
Author(s) -
Burkart Michael D.,
Izumi Masayuki,
Wong ChiHuey
Publication year - 1999
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/(sici)1521-3773(19990917)38:18<2747::aid-anie2747>3.0.co;2-2
Subject(s) - sulfotransferase , sulfation , chemistry , enzyme , biochemistry , cofactor , sulfate , recombinant dna , organic chemistry , gene
A cost‐efficient preparative enzymatic sulfation of oligosaccharides has been developed. Starting from adenosine 3′5′‐diphosphate (PAP), the sulfate donating and highly expensive cofactor 3′‐phosphoadenosine‐5′‐phosphosulfate (PAPS, 1 ) can be regenerated by using a recombinant aryl sulfotransferase and p ‐nitrophenyl sulfate. This system averts product inhibition by PAP and can serve as a continuous spectrophotometric assay for the activity of any sulfotransferase enzyme.