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Photoregulation of the Formation and Dissociation of a DNA Duplex by Using the cis – trans Isomerization of Azobenzene
Author(s) -
Asanuma Hiroyuki,
Ito Takanori,
Yoshida Takayuki,
Liang Xingguo,
Komiyama Makoto
Publication year - 1999
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/(sici)1521-3773(19990816)38:16<2393::aid-anie2393>3.0.co;2-7
Subject(s) - azobenzene , moiety , isomerization , oligonucleotide , chemistry , duplex (building) , dissociation (chemistry) , dna , stereochemistry , photochemistry , polymer , organic chemistry , biochemistry , catalysis
The duplex‐forming activity of an oligonucleotide has been photoregulated by making use of the isomerization of an azobenzene moiety in the side chain. When the azobenzene moiety is isomerized from the trans form to the cis form upon photoirradiation, the melting temperature of the duplex between the oligonucleotide and its complementary counterpart is significantly lowered, and the duplex is largely dissociated into two single‐stranded oligonucleotides (shown schematically).