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“Base Flipping”: Photodamaged DNA–RNA Duplexes Are Poor Substrates for Photoreactivating DNA‐Repair Enzymes
Author(s) -
Butenandt Jens,
Burgdorf Lars T.,
Carell Thomas
Publication year - 1999
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/(sici)1521-3773(19990301)38:5<708::aid-anie708>3.0.co;2-8
Subject(s) - dna , rna , photolyase , dna repair , base pair , chemistry , pyrimidine dimer , enzyme , biochemistry , biophysics , microbiology and biotechnology , stereochemistry , biology , gene
Abstract What is damaged cannot always be readily repaired. This is observed for particular areas in the genome (mutation hot spots), which are repaired with low efficiency. DNA–DNA duplexes that exist in the B‐conformation are repaired relatively efficiently by photolyases. DNA–RNA duplexes, which prefer an A‐type conformation are only moderately destabilized by DNA photolesions and are slowly repaired. This suggests that the DNA conformation modulates the necessary “flipping” process for the repair of DNA lesions.