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Induced Color Change of Conjugated Polymeric Vesicles by Interfacial Catalysis of Phospholipase A 2
Author(s) -
Okada Sheldon Yoshio,
Jelinek Raz,
Charych Deborah
Publication year - 1999
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/(sici)1521-3773(19990301)38:5<655::aid-anie655>3.0.co;2-4
Subject(s) - bathochromic shift , vesicle , conjugated system , chemistry , phospholipase a2 , catalysis , substrate (aquarium) , hydrolysis , biophysics , chemical modification , enzyme , chemical engineering , combinatorial chemistry , fluorescence , biochemistry , organic chemistry , membrane , polymer , biology , physics , quantum mechanics , ecology , engineering
A blue to red color change is induced on addition of phospholipase A 2 to modified PDA vesicles 1 (PDA=polydiacetylene). This bathochromic transition results from chemical modification of the vesicles by hydrolysis of the enzyme substrate embedded in the PDA matrix. Addition of a known phospholipase inhibitor or removal of Ca 2+ ions suppresses the color change, which suggests the potential for applications in high‐throughput screening assays.