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Chemo‐Enzymatic Synthesis of Fluorescent Rab 7 Proteins: Tools to Study Vesicular Trafficking in Cells
Author(s) -
Owen David J.,
Alexandrov Kirill,
Rostkova Elena,
Scheidig Axel J.,
Goody Roger S.,
Waldmann Herbert
Publication year - 1999
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/(sici)1521-3773(19990215)38:4<509::aid-anie509>3.0.co;2-3
Subject(s) - rab , vesicular transport protein , vesicle , microbiology and biotechnology , intracellular , enzyme , biochemistry , chemistry , biology , gtpase , membrane
The N ‐methylanthraniloylisoprenoid diphosphate derivatives 1 and 2 bind to RabGGTase II and are enzymatically transferred to Rab 7 (the Rab proteins are small G‐proteins that control events of docking and fusion of intracellular vesicles). The fluorescent Rab 7 proteins thus obtained may become important tools for further biological studies on vesicular trafficking in cells.