Premium
Bioaffinity NMR Spectroscopy: Identification of an E‐Selectin Antagonist in a Substance Mixture by Transfer NOE
Author(s) -
Henrichsen Dirk,
Ernst Beat,
Magnani John L.,
Wang WeiTong,
Meyer Bernd,
Peters Thomas
Publication year - 1999
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/(sici)1521-3773(19990115)38:1/2<98::aid-anie98>3.0.co;2-v
Subject(s) - antagonist , chemistry , nuclear magnetic resonance spectroscopy , identification (biology) , stereochemistry , biochemistry , receptor , biology , botany
Unambiguous identification of the E‐selectin antagonist 1 in a mixture of potential E‐selectin ligands is possible with a bioactivity assay that is based on transfer NOE. This is remarkable, since the compounds present in the mixture have similar structures and their 1 H NMR signals overlap extensively.