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Synthesis of Immobilized CMP‐Sialic Acids and Their Enzymatic Transfer with Sialyltransferase
Author(s) -
Kajihara Yasuhiro,
Ebata Takashi,
Kodama Hisashi
Publication year - 1998
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/(sici)1521-3773(19981204)37:22<3166::aid-anie3166>3.0.co;2-i
Subject(s) - sialyltransferase , sialic acid , glycoprotein , chemistry , enzyme , biochemistry , oligosaccharide , asialoglycoprotein receptor , in vitro , hepatocyte
A novel immobilization procedure for glycoproteins is based on the reacion of immobilized CMP‐NeuAc (CMP‐sialic acids) with sialyltransferase (see scheme). The transfer ability was tested under various conditions; a corresponding oligosaccharide or asialoglycoprotein acted as sialyl acceptor. The solid‐phase synthesis of CMP‐NeuAc is also described.